wt jurkat clone e6 1 cell line (ATCC)
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wt jurkat clone e6 1 cell line
Wt Jurkat Clone E6 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+jurkat+clone+e6+1+cell+line/Jurkat%2C+Clone+E6-1/bio_rxiv__2025__08__05__668721-211-0-10
Average 99 stars, based on 4439 article reviews
Wt Jurkat Clone E6 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+jurkat+clone+e6+1+cell+line/Jurkat%2C+Clone+E6-1/bio_rxiv__2025__08__05__668721-211-0-10
Average 99 stars, based on 4439 article reviews
wt jurkat clone e6 1 cell line - by Bioz Stars,
2026-09
99/100 stars
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1) Product Images from "FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling"
Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling
Journal: bioRxiv
doi: 10.1101/2025.08.05.668721
Figure Legend Snippet: (A) Flow cytometry analysis of tetrameric FGL-1 FD::streptavidin Phycoerythrin (SA-PE) binding to LAG-3+ Jurkat cells across a concentration range (1 – 16 µM). (B) Comparison of FGL-1 FD tetramer binding to LAG-3+ Jurkat cells versus wild-type (WT) Jurkat cells (LAG-3 low) (n = 3). Data are presented as median fluorescent intensity (MFI), normalized to LAG-3+ Jurkat cells MFI at each concentration. Error bars represent standard deviation (SD). (C) NF-κB::eGFP induction and (D) IL-2 production in LAG-3+ Jurkat T cells stimulated with anti-CD3/CD28-coated beads +/− FGL-1 FD or mouse IgG1 isotype control (mIgG1) for 24 hours (n = 4, n = 3, respectively). NF-κB::eGFP induction data is shown as percent MFI normalized to anti-CD3/CD28 stimulation (set to 100%). Error bars represent standard deviation (SD). (E) Expression of T cell activation markers: PD-1, CD69, CD25, ICOS, 4-1BB and OX40 in LAG-3+ Jurkat T cells, either unstimulated or stimulated with anti-CD3/CD28 +/− FGL-1 FD (n = 4). Data are shown as MFI ± SEM, normalized to the anti-CD3/CD28 condition. (F) Control expression of activation markers in WT (LAG-3 low) Jurkat T cells under identical conditions (n = 4). Data represent MFI ± SEM from triplicate wells across two independent experiments. Statistical significance was performed using one or two-way ANOVA, followed by Tukey or Šídák’s post hoc tests, respectively. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant p > 0.05.
Techniques Used: Flow Cytometry, Binding Assay, Concentration Assay, Comparison, Standard Deviation, Control, Expressing, Activation Assay
Figure Legend Snippet: (A, B) Label-free LC–MS/MS analysis of phosphotyrosine (pY) peptides in LAG-3+ Jurkat cells, either unstimulated or stimulated for 5 minutes with anti-CD3/CD28 +/− FGL-1 FD (n = 4), (A) number of unique pY peptides identified per condition (B) Log 2 intensity distribution of detected pY peptides. Statistical analysis performed using two-way ANOVA with Tukey’s post hoc tests. Solid black line = median; dashed lines = interquartile range. **** p < 0.0001, ns = not significant (p > 0.05) (C) Venn diagram showing overlap of pY peptides detected in cells stimulated with anti-CD3/anti-CD28 versus anti-CD3/anti-CD28 + FGL-1 FD from one representative experiment. (D – F) Volcano plots illustrating differential log 2 fold changes (log 2 FC) in pY intensity differences, across the three conditions: (D) anti-CD3/CD28 vs. unstimulated; (E) anti-CD3/CD28 + FGL-1 FD vs. unstimulated; (F) anti-CD3/CD28 + FGL-1 FD vs. anti-CD3/CD28. Red = significantly upregulated phosphosites (log 2 FC ≥ 1.5); blue = significantly downregulated phosphosites, (log 2 FC ≤ 1.5); grey = unchanged phosphosites (log 2 FC) < 1.5).
Techniques Used: Liquid Chromatography with Mass Spectroscopy
Figure Legend Snippet: ( A ) Functional enrichment analysis of proteins with upregulated pY sites (log 2 FC ≥ 1.5, red), and downregulated pY (log 2 FC ≤ 1.5; blue) in response to anti-CD3/CD28 and anti-CD3/CD28 + FGL-1 FD stimulation, relative to unstimulated cells and stimulation without FGL-1 FD. Color intensity indicates –Log 10 (p-value) of pathway enrichment; X denotes non-enriched pathways. ( B ) Log 2 fold change (log 2 FC) analysis of TCR signaling-associated pY peptides following anti-CD3/CD28 and anti-CD3/CD28 + FGL-1 FD stimulation compared to unstimulated cells. ( C ) Heatmap of log 2 FC phosphorylation differences in TCR signaling-related pY peptides across three conditions: anti-CD3/CD28 vs. unstimulated; anti-CD3/CD28 + FGL-1 FD vs. unstimulated; anti-CD3/CD28 + FGL-1 FD vs. anti-CD3/CD28. ( D ) Bar graphs showing log 2 FC values of TCR signaling pY sites in CD3 ITAMS, ZAP-70, LAT, ERK1/2, TEC and distal molecules upon FGL-1 FD stimulation relative to stimulation without FGL-1 FD. ( E ) Time-course western blot analysis of phosphorylation at CD3ζ Y142, ZAP70 Y319, and ERK 1/2 Y204/Y187 in LAG-3+ Jurkat cells unstimulated, stimulated with anti-CD3/CD28 +/− FGL-1 FD. Quantification represents phospho/total protein signal intensity, normalized to unstimulated (US) values (n = 3). ( F ) Bar graphs showing log 2 FC values of CD28 signaling pY sites: CD28 pY191, pY209, and PI3K P85A pY467, in response to FGL-1 FD stimulation relative to stimulation without FGL-1 FD. ( G ) Western blot analysis of CD28 Y191 phosphorylation in LAG-3+ cell lysates: unstimulated, stimulated with anti-CD3/CD28 +/− FGL-1 FD. Band intensities were quantified and normalized to total CD28 and unstimulated control (n = 5). Statistical significance was determined using a two-way ANOVA with Šídák’s post hoc tests. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant (p > 0.05).
Techniques Used: Functional Assay, Phospho-proteomics, Western Blot, Control
Figure Legend Snippet: ( A ) Representative fixed-cell confocal microscopy images showing colocalization of CD3 (green) and LAG-3 (red) in unstimulated and anti-CD3/CD28-stimulated LAG-3+ Jurkat cells. Scale bar: 5 µm. (B) Representative confocal images depicting colocalization of CD28 (green) and LAG-3 (red) under the same conditions: unstimulated and anti-CD3/CD28. Scale bar: 5 µm. (C) Violin plots of Manders’ Overlap Coefficient (MOC) quantifying colocalization of CD3 and CD28 with LAG-3 in unstimulated and stimulated cells (n = 66). Median values are indicated by solid black lines; interquartile ranges by dashed black lines. Statistical significance was assessed using Kruskal-Wallis ANOVA, followed by Dunnett’s post hoc tests. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant (p > 0.05).
Techniques Used: Confocal Microscopy
Figure Legend Snippet: ( A ) Representative fixed-cell confocal microscopy images showing colocalization of CD28 (red) and Lck (pY394, cyan) in unstimulated, anti-CD3/CD28-stimulated, and anti-CD3/CD28 +/− FGL-FD stimulated LAG-3+ Jurkat cells. Scale bar: 5 µm. (B) Violin plots quantifying CD28 and Lck pY394 colocalization using Manders’ overlap coefficient (MOC) and Pearson’s correlation coefficient (PCC) (n ≥ 50). (C) Representative confocal images showing colocalization of CD28 (green) and total Lck (red) in unstimulated, anti-CD3/CD28 +/− FGL-FD stimulated LAG-3+ Jurkat cells. Scale bar: 5 µm. (D) Colocalization analysis of CD28 and total Lck, measured by MOC and PCC (n ≥ 60). (E) Representative confocal images of CD28 (red) and Lck pY394 (cyan) in unstimulated, anti-CD3/CD28 +/− FGL-FD stimulated Jurkat cells. Scale bar: 5 µm. (F) Colocalization analysis of CD28 and Lck pY394, determined by MOC and PCC (n ≥ 75). (G) Comparison of MOC and PCC values for CD28 and Lck pY394 colocalization between unstimulated and stimulated LAG-3+ Jurkat cells compared to WT (LAG-3 low) Jurkat cells (n ≥ 75). Median values are shown in solid black lines; interquartile ranges as dashed black lines. Statistical analysis was performed using Kruskal-Wallis ANOVA test, followed by Dunnett’s post hoc tests. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = not significant (p > 0.05).
Techniques Used: Confocal Microscopy, Comparison
Related Articles
Flow Cytometry:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Binding Assay:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Concentration Assay:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Comparison:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Standard Deviation:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Control:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Expressing:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Activation Assay:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Liquid Chromatography with Mass Spectroscopy:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Functional Assay:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Phospho-proteomics:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Western Blot:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. Confocal Microscopy:Article Title: FGL-1 binding to LAG-3 inhibits T cell activation via disruption of CD28 and TCR signaling Article Snippet: LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).LAG-3+ NF-κB::eGFP reporter Jurkat E6.1 T cells were a gift from Jun Wang (NYU).. |